Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Early life acetaminophen exposure, glutathione S transferase genes, and development of adolescent asthma in a high risk birth cohort Journal of Allergy and Clinical Immunology Glutathione Benefits for Better Thyroid Health Paloma Health Glutathione IV for Liver Detox: Support & Benefits AZ IV Medics Researchers are studying a possible link between acetaminophen (Tylenol) use during pregnancy and higher autism or ADHD risk. It may lower glutathione, an important antioxidant for brain development. Tylenol Liver Damage Signs, Causes, Dosage, Stages & Prognosis